Timetable for Porecamp 2016
All group activities (coffee, lectures and reportage) and bioinformatics practicals will be held in the Exchange, Penryn Campus (Cornwall), University of Exeter.
Monday 15th August: Introduction
- 12:30 : Registration and welcome buffet in Yellow Seminar Room
- 14:30 : Introduction to Porecamp (Konrad Paszkiewicz)
- 14:50 : Laboratory Safety (MD Sharma)
- 15:00 : Participants and their projects (Matt Loose)
- 15:30 : Overview of the MinION platform (Josh Quick)
- 16:15 : Making a good library (Justin O’Grady)
- 16:45 : Break and informal discussion on perfect MinION experiment
- 17:30 : Group discussion on your perfect MinION experiment? (Matt Loose)
- 18:00 : Settle into accommodation (collect keys from Glasney Lodge)
- 19:30 : Group dinner on campus (Lower Stannary room in the Daphne Du Maurier building)
Tuesday 16th August: Practicals
Rotations: Group A Library Prep, Group B MinION Running, Group C Bioinformatics
- 09:00 : Group update in Yellow Seminar Room
- 09:15 : Practicals
- 13:00 : Lunchtime seminar: Understanding event calling and MinION data (Matt Loose)
- 14:00 : Practicals (continued)
Wednesday 17th August: Practicals
Rotations: Group A Bioinformatics, Group B Library Prep, Group C MinION Running
- 09:00 : Group update in Yellow Seminar Room
- 09:15 : Practicals
- 13:00 : Lunchtime seminar: MinION Running (John Tyson)
- 14:00 : Practicals (continued)
Thursday 18th August: Practicals
Rotations: Group A MinION Running, Group B Bioinformatics, Group C Library Prep
- 09:00 : Group update in Yellow Seminar Room
- 09:15 : Practicals
- 13:00 : Lunchtime seminar: Utilising nanopore sequencing for biothreat detection (Claire Lonsdale)
- 14:00 : Practicals (continued)
Friday 19th August: Data analysis and conclusions
- 09:00 : Group update in Yellow Seminar Room
- 09:15 : Seminar: Deploying a portable nanopore sequencing system (Josh Quick)
- 10:15 : Lab practical: Bioinformatics continued
- 13:00 : Lunch and wrap up (Nick Loman & Konrad Paszkiewicz)
Teaching materials
Shared results
Bioinformatics tutorials
- Nick’s bioinformatics lectures
- Logging onto CLIMB server desktop with X2GO
- 00. Using the Linux command-line
- 01. Using CLIMB
- 02. Understanding your MinION data
- Example nanook report
- 03. Mapping and variant calling on Ebola
- 04. De novo and hybrid assembly
- Barcode demultiplexing
- Porecamp virtual machine